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Parameters used in simulations of Ca2+ spark formation and detection as shown in Fig 3.2.

Local calcium release from the SR

Parameter	definition	value	unit
iCa	Amplitude of elemental Ca2+ release	2	pA
F	Faraday's constant	96500	C/mol
Z	Valence of Ca2+ ion	2

Diffusion of free CA2+ and fluo-3

Parameter	definition	value	unit
Dc	Diffusion coefficient of free Ca2+ in cytosol	250	µm^2 s^-1
Db	Diffusion coefficient of fluo-d and Ca2+-fluo-3	20(varied)	µm^2 s^-1

Diffusion of free CA2+ and fluo-3

Parameter	definition	value	unit
vpump-max	Maximum rate of Ca2+ reuptake	208	µM / s
Kpump	Michaelis constant	0.184	µM
m	Hill coefficient	3.9

Diffusion of free CA2+ and fluo-3

Parameter	definition	value	unit
FWHMz	Axial full width at half-maximum	0.8	µm
FWHMxy	Lateral full width at half-maximum	0.8	µm

Miscellaneous

Parameter	definition	value	unit
[Ca2+]inf	Intracellular free Ca2+ at rest	0.1	µM

Modified from Smith, G.D., Keizer, J., Stern, M., Lederer, W.J., and Cheng, H.,, Biophys. J., 75, 15, 1998. with permission